ROTTERDAM, Netherlands — Top-seeded Rafael Nadal lost to Andreas Seppi of Italy 3-6, 6-3, 6-4 on a day of upsets today, leaving the ABN Amro without seeded players entering the quarterfinals.

Beaten earlier in the day were second-seeded Nikolay Davydenko of Russia, third-seeded David Ferrer of Spain and fifth-seeded Tomas Berdych of the Czech Republic.

It is only the third time since 2000 no seeded players are in the quarters of an ATP tournament. It previously happened in 2006 in Nottingham, England, and in 2002 in Buenos Aires, Argentina.

Seppi, playing on his 24th birthday, eliminated the No. 2-ranked Spaniard and the three-time French Open champion.

"There is no better way to celebrate your birthday," Seppi said. "This was one of my best matches ever because I served really well."

Seppi, who beat Lleyton Hewitt in the first round, will now have to change his travel plans — again.

"I had booked a flight for Wednesday night and had to change it yesterday," he said. "After this win I'll wait until rebooking my flight home."

Davydenko lost 6-3, 7-5 to Michael Llodra of France; Ferrer fell 6-2, 7-5 to Mischa Zverev of Germany; and Berdych lost 6-4, 6-4 to Ivo Karlovic of Croatia.

Nadal broke Seppi twice to take the opening set, but the Italian rebounded to win the second. Down two breaks in the third set, Nadal saved two match points in the eighth game and then broke on his fourth break point.

Nadal held serve at love in the next game, but Seppi closed the match by holding at love. Nadal challenged the call on his final forehand, but replays confirmed it was long and Seppi raised both fists in triumph.

Llodra converted all three break-point opportunities. Davydenko, who capitalized on only one of eight, received treatment for a shoulder injury in the second set.

Ferrer smashed two rackets in his loss to Zverev, who reached his third quarterfinal. Zverev broke twice in each set while dropping serve only once, in the second set.

"I expected to be sitting at home in Hamburg tonight," Zverev said. "This is the biggest win of my career."

Zverev will play Karlovic, who had 17 aces against Berdych.